Figure 1: CoMIC appears in elongated mitochondria before mitochondrial division.
From: Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division
(a) Time-lapse images of mitochondrial matrices (DsRed-mito) in neuronal process (GFP). The white arrowheads and the arrow indicate sites of mitochondrial bulging and division, respectively. Scale bars, 10 and 5 μm in the lower and higher magnification images, respectively. (b) Kymograph of DsRed-mito in a. The lower graph is the intensity profile at two CoMIC-showing time points (1, 2), and arrow heads indicate conserved bulging site. Two illustrations on the right indicate the pattern of CoMIC and occurrence of division. CoMIC pattern and division are labelled by spikes and an arrow, respectively. (c) Kymograph of DsRed-mito in a neuronal process. The upper numbers indicate mitochondria showing CoMIC. The lower panels show the pattern of CoMIC and occurrence of division in mitochondria labelled with the numbers above. (d) Probability of CoMIC in mitochondria from young (DIV4; <5 μm=530 mitochondria, >5 μm=70 mitochondria) and mature neurons (DIV10; <5 μm=114 mitochondria, >5 μm=111 mitochondria) during a 10-min time period (***P<0.01 by the Mann–Whitney rank sum test). Error bars indicate s.e. (e) Proportion of mitochondria undergoing subsequent division among mitochondria showing no or prior CoMIC (***P<0.01 by the χ2-test with Yates’s correction for continuity). (f) Proportion of mitochondria showing prior CoMIC among non-dividing or dividing mitochondria (***P<0.01 by χ2-test with Yates’s correction for continuity).
