Figure 5: Intra-mitochondrial Ca2+ regulates CoMIC.
From: Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division
(a) Time-lapse images of DsRed-mito and CEPIA3mt in DN-Drp1-expressing neuron. Arrow heads indicate mitochondrial bulging. Scale bar, 5 μm, and CEPIA3mt/DsRed-mito is pseudo-coloured. (b) Kymographies of DsRed-mito and CEPIA3mt/DsRed-mito of mitochondria in dotted box of a. (c) Temporal CEPIA3mt/DsRed-mito fluctuation of mitochondria of dotted boxes (#1, #2) of b. Upper red boxes indicate CoMIC duration of mitochondrion labelled by #2. (d) Representative kymographies of mito-GFP and patterns of CoMIC (right diagram) in DN-Drp1-expressing neurons treated with DMSO and 2-APB (1 μM) for 1 h. (e) Probability of CoMIC in elongated mitochondria treated with DMSO (103 mitochondria) and 2-APB (94 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC (***P<0.01 and *P<0.05 by Mann–Whitney rank sum test). Error bar indicate s.e. (f–i) Representative kymographies of DsRed-mito and patterns of CoMIC (right diagram) in DN-Drp1-expressing neurons treated with DMSO (f), Ru360 (10 μM) (g), thapsigargin (TG, 1 μM) (h) and TG with 30 min pretreatment with Ru360 (Ru360+TG) (i) for 1 h. (j) Probability of CoMIC in elongated mitochondria treated with DMSO (100 mitochondria), Ru360 (95 mitochondria), TG (88 mitochondria) or Ru360+TG (95 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC (***P<0.01, **P<0.02 and *P<0.05 by Mann–Whitney rank sum test). (k) Representative kymographies of DsRed-mito and patterns of CoMIC (right diagram) in DN-Drp1+shMock- and DN-Drp1+shMcu-expressing neurons. (l) Probability of CoMIC in elongated mitochondria from DN-Drp1+shMock- (105 mitochondria) and DN-Drp1+shMcu-expressing neurons (118 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC (**P<0.02 and *P<0.05 by Mann–Whitney rank sum test).
