Figure 8: Oma1-mediated cleavage of Opa1 regulates CoMIC.
From: Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division
(a–d) Representative kymographies of DsRed-mito and patterns of CoMIC (right diagram) in DN-Drp1+shMock- (a), DN-Drp1+shOpa1- (b), TG-treated DN-Drp1+shMock- (c) or TG-treated DN-Drp1+shOpa1-expressing neurons (d). (e) Probability of CoMIC in elongated mitochondria from DN-Drp1+shMock-, DN-Drp1+shOpa1- (123 mitochondria), TG-treated DN-Drp1+shMock- (138 mitochondria) and TG-treated DN-Drp1+shOpa1-expressing neurons (105 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC (***P<0.01, **P<0.02 and *P<0.05 by Mann–Whitney rank sum test). Error bar indicate s.e. (f) Representative kymography of DsRed-mito and patterns of CoMIC (right diagram) in DN-Drp1+shOma1-expressing neuron. (g) Probability of CoMIC in elongated mitochondria from DN-Drp1+shMock- and DN-Drp1+shOma1-expressing neurons (134 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC (**P<0.02 and *P<0.05 by Mann–Whitney rank sum test). (h–m) Representative kymographies of DsRed-mito and patterns of CoMIC (right diagram) in DN-Drp1- (h), DN-Drp1+S-Opa1- (i), ruthenium Red (RuR, 10 μM)-treated DN-Drp1+S-Opa1- (j), Pax-treated DN-Drp1+S-Opa1- (k), DN-Drp1+L-Opa1- (l) and TG-treated DN-Drp1+L-Opa1-expressing neurons (m). (n) Probability of CoMIC in elongated mitochondria from DN-Drp1- (105 mitochondria), DN-Drp1+S-Opa1- (111 mitochondria), RuR-treated DN-Drp1+S-Opa1- (105 mitochondria) and Pax-treated DN-Drp1+S-Opa1-expressing neurons (104 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC (***P<0.01, **P<0.02 and *P<0.05 by Mann–Whitney rank sum test). (o) Probability of CoMIC in elongated mitochondria from DN-Drp1-, TG-treated-DN-Drp1- (138 mitochondria), DN-Drp1+L-OPA1- (140 mitochondria) and TG-treated DN-Drp1+L-Opa1-expressing neurons (104 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC. (***P<0.01 and **P<0.02 by Mann–Whitney rank sum test).
