Figure 5: Combinatorial loss of Vhl and Ink4a/Arf with MYC activation promotes metastasis and activation of EMT genes.
From: MYC activation cooperates with Vhl and Ink4a/Arf loss to induce clear cell renal cell carcinoma
(a) A representative H&E stained liver sections from a VIM mouse shows metastasis to the liver parenchyma as well as intravascular mets. Higher power magnification reveals features resembling renal clear cell carcinomas within the metastatic lesions. (Scale bar, 100 μm). (b) Bar graph showing percentage of mice with metastases. (c) Heat map showing the expression patterns of EMT associated genes in VM-3055 and VIM-3039 cells. (d) Higher expression of genes involved in invasion and metastasis is observed in VIM-3039 cells compared to VM-3055 cells. (e) Matrigel invasion assays show increased invasion from VIM-3039 cells compared to VM-3055 cells. ****P<0.0001. (d,e) P values obtained from student t-test. Data are presented as mean ±s.e.m.
