Figure 6: Restoration of VHL does not significantly reverse tumorigenic capacity of VIM cells.
From: MYC activation cooperates with Vhl and Ink4a/Arf loss to induce clear cell renal cell carcinoma
(a) Immunoblot of whole cell lysates from VIM cells infected with pBabe eGFP and pBabe HA-VHL. (b) Cell growth assay shows no significant reduction in cell number in VIM cells when VHL is expressed. Cells grown on Dox or vehicle were analysed in triplicate each day. (c) Soft agar assays show VIM-3039 cells form colonies in an anchorage independent manner despite the presence of VHL. (b,c) P values obtained from student t-test. Data are presented as mean ±s.e.m.
