Figure 5: Global transcriptional effects of KPT-6566.
From: A covalent PIN1 inhibitor selectively targets cancer cells by a dual mechanism of action
(a) Venn diagram of the Illumina microarray analysis with common and differentially up- and down-regulated genes of MDA-MB-231 cells treated with siRNA PIN1#1 vs. KPT-6566, using a cutoff of 0.75 logFC and P-value ≤0.05. (b) Venn diagram of the Gene Ontology (GO) analysis with common and differential enrichment for functional terms of MDA-MB-231 cells treated with siRNA PIN1 vs. KPT-6566. (c) Histogram indicating comparison of IPA analysis of genes regulated by PIN1 siRNA (white) or KPT-6566 (red) in function of their activation Z-scores. (d) Quantitative RT-PCR analysis of NRF2 pathway members in MDA-MB-231 cells treated with the indicated compounds for 48 h. Data are indicated as means±s.d. of n=3 independent experiments, *P<0.05, **P<0.01; two-tailed Student’s t-test.
