Figure 6: KPT-6566 induces DNA damage in a PIN1-dependent manner.
From: A covalent PIN1 inhibitor selectively targets cancer cells by a dual mechanism of action
Immunoblotting of the indicated proteins from (a) MDA-MB-231 cells treated with increasing amounts of KPT-6566 or DMSO for 48 h; (b) MCF10A, MDA-MB-231 and MDA-MB-468 cells treated with 5 μM KPT-6566 (+), 10 μM Bleomycin (+) or DMSO (−) for 48 h; (c) MDA-MB-231 cells transfected with control- (siRNA Ctrl) or two different PIN1 siRNAs (siRNA PIN1#1 and PIN1#2) and treated as indicated for 24 h. (d) Histogram representing the percentage of γH2AX positive nuclei of MDA-MB-231 cells treated as in c. (e) Immunoblotting of the indicated proteins from MDA-MB-231 cells treated with the indicated compounds (+) or DMSO (–) for 48 h. a–c,e Actin levels are reported as loading control; size markers are indicated. Data shown in (d) are the means±s.d. of n=3 independent experiments, *P<0.05, **P<0.01; two-tailed Student’s t-test.
