Figure 2: ILC2 populations are decreased during high fat diet.
From: Type-2 innate lymphoid cells control the development of atherosclerosis in mice
Spleen, bone marrow (BM), mesenteric lymph node (MLN, shown) and para-aortic lymph node (PaLN, shown) from Ldlr−/−mice maintained on high fat diet (HFD) for 8 weeks were analysed for ILC2 populations by flow cytometry (a). While BM resident ILC2 were unchanged (top left) a downward trend in splenic ILC2 and a statistically significant decrease in ILC2 was observed in MLN and PaLN (b). ELISA analysis of IL-5 and IL-13 in the supernatants of sorted and cultured ILC2 (1 × 105 cells per well) also demonstrated a decrease in cytokine secretion (c). QPCR on sorted splenic, aortic and GWAT ILC2 from Ldlr−/−mice maintained on chow or high fat diet (HFD) for 8 weeks. Data from aortic ILC2 represent two pools of three mice each. Each other square or triangle represents data from one separate mouse (d). Statistical significance was determined by Mann–Whitney U-test.
