Figure 1: Engineering and in vitro characterization of a high-fidelity base editor (HF-BE3). | Nature Communications

Figure 1: Engineering and in vitro characterization of a high-fidelity base editor (HF-BE3).

From: Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

Figure 1

(a) Schematic representation of HF-BE3. Point mutations introduced into BE3 to generate HF-BE3 are shown in green. The representation used PDB structures 4UN3 (Cas9), 4ROV (cytidine deaminase) and 1UGI (uracil DNA glycosylase inhibitor). (b) In vitro deamination of synthetic substrates containing ‘TC’ repeat protospacers. Values and error bars reflect the mean and range of two independent replicates performed on different days.

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