Figure 2: Activity of a high-fidelity base editor (HF-BE3) in human cells. | Nature Communications

Figure 2: Activity of a high-fidelity base editor (HF-BE3) in human cells.

From: Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

Figure 2

(a–c) On- and off-target editing associated with plasmid transfection of BE3 and HF-BE3 was assayed using high-throughput sequencing of genomic DNA from HEK293T cells treated with sgRNAs targeting non-repetitive genomic loci EMX1 (a), FANCF (b) and HEK293 site 3 (c). On- and off-target loci associated with each sgRNA are separated by a vertical line. (d) On- and off-target editing associated with the highly repetitive sgRNA targeting VEGFA site 2. Values and error bars reflect mean±s.d. of three independent biological replicates performed on different days. For a–c, stars indicate significant editing based on a comparison between the treated sample and an untreated control. *P≤0.05, **P≤0.01 and ***P≤0.001 (Student’s two-tailed t-test). For d, asterisks are not shown since all treated samples displayed significant editing relative to the control. Individual P values are listed in Supplementary Table 1.

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