Figure 3: Protein delivery of base editors into cultured human cells. | Nature Communications

Figure 3: Protein delivery of base editors into cultured human cells.

From: Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

Figure 3

(a–d) On- and off-target editing associated with RNP delivery of base editors complexed with sgRNAs targeting EMX1 (a), FANCF (b), HEK293 site 3 (c) and VEGFA site 2 (d). Off-target base editing was undetectable at all of the sequenced loci for non-repetitive sgRNAs. Values and error bars reflect mean±s.d. of three independent biological replicates performed on different days. Stars indicate significant editing based on a comparison between the treated sample and an untreated control. *P≤0.05, **P≤0.01 and ***P≤0.001 (Student’s two-tailed t-test).

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