Figure 4: Effect of dosage of BE3 protein or plasmid on the efficiency of on-target and off-target base editing in cultured human cells. | Nature Communications

Figure 4: Effect of dosage of BE3 protein or plasmid on the efficiency of on-target and off-target base editing in cultured human cells.

From: Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

Figure 4

(a) On-target editing efficiency at each of the four genomic loci was averaged across all edited cytosines in the activity window for each sgRNA. Values and error bars reflect mean±s.e.m. of three independent biological replicates performed on different days. (b,c) On- and off-target editing at the EMX1 site arising from BE3 plasmid titration (b) or BE3 protein titration (c) in HEK293T cells. Values and error bars reflect mean±s.d. of three independent biological replicates performed on different days.

Back to article page