Figure 5: DNA-free in vivobase editing in zebrafish embryos and in the inner ear of live mice using RNP delivery of BE3. | Nature Communications

Figure 5: DNA-free in vivobase editing in zebrafish embryos and in the inner ear of live mice using RNP delivery of BE3.

From: Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

Figure 5

(a) On-target genome editing in zebrafish harvested 4 days after injection of BE3 complexed with indicated sgRNA. Values and error bars reflect mean±s.d. of three injected and three control zebrafish. Controls were injected with BE3 complexed with an unrelated sgRNA. (b) Schematic showing in vivo injection of BE3:sgRNA complexes encapsulated into cationic lipid nanoparticles. (c) Base editing of cytosine residues in the base editor window at the VEGFA site 2 genomic locus. (d) On-target editing at each cytosine in the base-editing window of the VEGFA site 2 target locus. (c,d) Values and error bars reflect mean±s.e.m. of three mice injected with sgRNA targeting VEGFA site 2, three uninjected mice and one mouse injected with unrelated sgRNA.

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