Figure 2: In vivo silencing of Ash1l renders mice more susceptible to experimental colitis. | Nature Communications

Figure 2: In vivo silencing of Ash1l renders mice more susceptible to experimental colitis.

From: Ash1l and lnc-Smad3 coordinate Smad3 locus accessibility to modulate iTreg polarization and T cell autoimmunity

Figure 2

(a–c) TNBS-colitis was induced in 8-week-old WT and Ash1l-silenced mice (n=5 mice per group) and monitored for disease development for 3 days after TNBS induction. (a) Weight change over time of WT and Ash1l-silenced mice after TNBS induction, presented relative to weight at day 0, set as 100%. (b,c) Appearances (b) of colons, reduction of colon length (c) were examined at day3 after TNBS induction. (d–f) T cell adoptive transfer-mediated colitis was induced in 6-week-old Rag1−/− mice via transferring with WT CD4+CD25− T cells with or without WT or Ash1l-silenced iTreg cells (n=5 mice per group). Disease development was monitored for 6 weeks. (d) Weight change over time of Rag1−/− mice transferred with WT CD4+CD25− T cells with or without WT or Ash1l-silenced iTreg cells, presented relative to weight at week 1, set as 100%. (e,f) Appearances (e) and length (f) of colons were examined at week 6 after T cell transfer. Error bars represent s.d. Student’s t test. *P<0.05, **P<0.01. Data are from three independent experiments (a,c,d,f; mean±s.d. of five mice) or are representative of three independent experiments (b,e).

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