Figure 1: The impact of Ubp2 on Fzo1 is not mediated by Rsp5. | Nature Communications

Figure 1: The impact of Ubp2 on Fzo1 is not mediated by Rsp5.

From: An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion

Figure 1

(a–f) MW in kDa are shown on the right or left of short and long exposures of indicated regions of immunoblots. (a) Total protein extracts prepared from WT and ubp2Δ cells (W303 background) were analysed by anti-Dnm1, anti-Fzo1, anti-Ugo1 and anti-Mgm1 immunoblotting. The localization of proteins is indicated on the right. (b) Total protein extracts prepared from WT, mdm30Δ and ubpXΔ cells (BY4741 background) transformed with pRS416-TEF-FZO1-13MYC were analysed by anti-Myc immunoblotting. Loading was normalized to Fzo1-13Myc levels. (c) Total protein extracts prepared from WT, rsp5Δ+spt23*, ubp2Δ and rsp5Δ+spt23* ubp2Δ cells (DF5 background) transformed with pRS414-TEF-FZO1-13MYC were analysed by anti-Myc and anti-Rsp5 immunoblotting. Loading was normalized to Fzo1-13Myc levels. (d) CHX chase analysed by anti-Fzo1 and anti-Pgk1 immunoblotting with strains used in c but free of any plasmid. (e) Total protein extracts prepared from WT, ubp2Δ and rup1Δ cells (BY4741 background) were analysed by anti-Fzo1 and anti-Pgk1 immunoblotting. (f) Total protein extracts prepared from cells used in e but transformed with pRS414-TEF-FZO1-13MYC were analysed by anti-Myc immunoblotting. Loading was normalized to Fzo1-13Myc levels. Sol, soluble; OM, outer membrane; IM, inner membrane; IMS, inter membrane space; l-Mgm1, long Mgm1; s-Mgm1, short Mgm1.

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