Figure 4: ERβ tissue profiling using antibody PPZ0506.
From: Insufficient antibody validation challenges oestrogen receptor beta research
IHC profiling of normal tissues (top panel) and cancer tissues (lower panel). Nuclear ERβ positivity using PPZ0506 (dilution 1:600) is identified in normal tissues of (a) tonsil, (b) peripheral inflammatory cells (small intestine), (c) testis and (d) ovary. No IHC positivity was seen in (e) breast, (f) liver, (g) kidney, or (h) prostate. Nuclear ERβ positivity is identified in cancerous tissues (i–l) three out of four granulosa cell tumours, (m) one case of melanoma, and (p) one case of thyroid cancer. The remaining 16 cancer types were all IHC-negative, here exemplified by (n) colorectal cancer and (o) breast cancer. Brown: positive IHC staining; Blue: hematoxylin counter staining. BC: breast cancer. Scale bar indicates 50 μm. Arrows indicate examples of cells with nuclear staining.
