Figure 7: PLTS interneurons receive synaptic input from THINs.
From: Differential processing of thalamic information via distinct striatal interneuron circuits
(a) Schematic of experimental preparation. (b) Responses of a typical PLTS interneuron to injected current pulses. (c) Optogenetic activation of THINs evokes an IPSP in a PLTS interneuron (n=27/32 recorded PLTS) that is blocked by 10 μM bicuculline (n=11). (d) Brief train (5 2 ms pulses at 20 Hz) evokes short-term depression in the IPSC that is blocked by bicuculline (n=9) (Vh=−45 mv). (e) Box plots showing mean amplitudes of the IPSC and IPSP. (f) Optogenetic stimulation of TH interneurons inhibits spontaneous firing of a PLTS interneuron (n=13). Lower panel: higher-resolution traces of the portion of spike train above indicated by the dashed line to show IPSP. (g) Upper: after injecting a 50 pA current pulse that induces firing, optogenetic activation of THINs (blue bar) induces a brief pause in firing. Lower: with 100 pA current pulse, the PLTS interneuron enters depolarization block that is relieved by optogenetic activation of THINs. (h) Optogenetic inhibition of a spontaneously active PLTS interneuron is reversibly blocked by bicuculline. Box plots represent the minimum, maximum interquartile range, the mean and median.
