Figure 6: β-catenin and TGF-β signalling overactivation mediates miR-128-3p-induced aggressiveness in NSCLC cells. | Nature Communications

Figure 6: β-catenin and TGF-β signalling overactivation mediates miR-128-3p-induced aggressiveness in NSCLC cells.

From: Simultaneous overactivation of Wnt/β-catenin and TGFβ signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC

Figure 6

(a and b) Effects of introducing constitutively active β-catenin (Δβ-catenin) and SMAD3 (CA-SMAD3) mutants, alone or in combination, on cell migration and tumour cell sphere growth in A549-luc-CDDP-4th (A) and A549 (B) cells transfected with NC or miR-128-3p inhibitor (Anti-miR). (c) Cell viability assays determining the resistance or sensitivity of A549-luc-CDDP-4th cells treated as indicated to CDDP. (d and e) Mice bearing tumour xenografts by subcutaneous inoculation (d) or experimental metastasis by intravenous injection (e) of A549-luc-CDDP-4th cells transduced with the constitutively active β-catenin and SMAD3 mutants, alone or in combination, were treated by intravenous administration of miR-128-3p antagomir, or by intraperitoneal injection of with ICG-001 and LY2157299, alone or in combination (Comb). Bioluminescent images of subcutaneous tumours and spontaneous/experimental metastasis are shown. (f) Tumour growth curves of A549-luc-CDDP-4th cells with the indicated pretreatments in mice intravenously administered miR-128-3p antagomir and CDDP in combination. (g) Tumour growth curve of A549-luc-CDDP-4th cells in mice intraperitoneally injected with the indicated inhibitors. (h) Immunostaining of β-catenin, SMAD3, E-cadherin, Vimentin and CD34 in tumour tissues of the indicated A549-luc-CDDP-4th cell xenografts. Scale bar: 25 μm. Error bars represent mean±s.d. derived from three independent experiments. A two-tailed Student’s t-test was used for statistical analysis (*P<0.05).

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