Figure 2: Spontaneous activation of conventional T cells in Foxp3^CreLkb1^f/f mice.

(a) Expression of CD44 and CD62L on splenic CD4⁺Foxp3⁻ and CD8⁺Foxp3⁻ T cells from Foxp3^Cre and Foxp3^CreLkb1^f/f mice. (b) Expression of Ki67, CD25 and CD69 in splenic CD4⁺Foxp3⁻ and CD8⁺Foxp3⁻ T cells from Foxp3^Cre and Foxp3^CreLkb1^f/f mice. (c) Intracellular staining of cytokines in splenic CD4⁺Foxp3⁻ and CD8⁺Foxp3⁻ T cells from Foxp3^Cre and Foxp3^CreLkb1^f/f mice stimulated with phorbol myristate acetate (PMA) and ionomycin for 4 h. All mice analysed were 28–30 days old. All data are representative of at least three independent experiments.