Figure 5: TRPV1 promotes excitatory innervation of OLM neurons.
From: TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus
(a) Quadruple immunostains of WT and TRPV1 KO hippocampal neurons with vGluT1 to assay excitatory presynaptic innervation, somatostatin and reelin to identify OLM cells, and MAP2 to identify neuronal somas and dendritic processes, in the indicated conditions. Merge indicates combination of vGluT, reelin and MAP-2 channels; scale bar, 20 μm. (b) Quantitation of excitatory synapse (vGluT1 puncta) number, in the indicated conditions, normalized to surrounding non-TRPV1-expressing cells. TRPV1 KO OLM neurons have reduced excitatory innervation, compared to WT (n=20 images for all conditions from three cultures). (c) Quadruple immunostain of WT and TRPV1 KO hippocampal neurons for vGAT, to mark inhibitory synapses, reelin, somatostatin, and MAP2. Merge indicates combination of vGAT, reelin and MAP2 channels; scale bar, 20 μm. (d) Quantitation of inhibitory synapse (vGAT puncta) number, in the indicated conditions, normalized to surrounding non-TRPV1-expressing cells (n=20 images for all conditions from 2 cultures). Error=s.e.m., statistical significance determined by one-way ANOVA with Tukey's post hoc test for multiple comparisons, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.
