Figure 1: Primary CD4 T cells from HTLV-1-infected individuals secrete LTB4.
From: HTLV-1-induced leukotriene B4 secretion by T cells promotes T cell recruitment and virus propagation
(a) PBMCs from HTLV-1-infected individuals secrete significantly more LTB4 than PBMCs from HDs. PBMCs were culture for 30 min and spontaneous LTB4 secretion levels were determined by ELISA. Dashed line represents the detection limit of the ELISA kit. PBMCs were from HDs (n=5), HACs (n=5) or individuals who had developed HAM/TSP (n=5). Bars represent mean. P value=0.03; Mann–Whitney U-test (HDs versus HACs and HAM/TSP). (b) CD4 T cells from HTLV-1-infected individuals secrete detectable levels of LTB4. CD4 T cells were isolated, cultured for 30 min and spontaneous LTB4 secretion was detected by ELISA. Bars represent mean. P value<0.001; Mann–Whitney U-test (HDs versus HACs and HAM/TSP). (c) LTB4 spontaneous secretion by CD4 T cells correlates with the percentage of cells infected with HTLV-1. Pearson’s correlation test: r=0.87; R2=0.76; P value <10−4.
