Figure 3: Tax-induced pla2g4c expression depends on both NF-κB and CREB pathways.
From: HTLV-1-induced leukotriene B4 secretion by T cells promotes T cell recruitment and virus propagation
(a) Tax mutants fail to activate pla2g4c promoter. Pla2g4c promoter was cloned into a luciferase reporter plasmid. 293T cells were transfected with the reporter plasmid (or the empty vector) and plasmids encoding Tax wild type (WT) or the mutants M22 (that do not activate the NF-κB pathway) or M47 (that do not activate the CREB pathway). Protein expression levels were detected by western blot 24 h post transfection (right panel). Luciferase activity (relative light units, RLU) was normalized to the protein concentration. P value=0.001; n=4; mean±s.e.m.; Two-way ANOVA, Sydak’s post hoc test. (b) The CRE and κB sites are both required for Tax-induced pla2g4c transactivation. 293T cells were transfected with a plasmid encoding Tax and with reporter plasmids with the luciferase gene downstream the WT pla2g4c promoter, or promoters depleted for the CRE or the κB sites (generated by site-directed mutagenesis). P value <10−4; n=4; mean±s.e.m.; Two-way ANOVA, Sydak’s post hoc test.
