Figure 1: Identification of distinct populations of AMPAR assemblies in the rat brain.
From: AMPA-receptor specific biogenesis complexes control synaptic transmission and intellectual ability
(a) Heat map indicating the molar abundance of AMPAR constituents determined in APs with various ABs targeting GluA1-4 (mixture of anti-GluA ABs), FRRS1l (anti-FRRS1l-a, anti-FRRS1l-b) and TARPs 2, 3, 4, 8 (anti-TARP-a, anti-TARP-b, anti-TARP-c) in membrane fractions from total rat brain solubilized with CL-47. Note the distinct subgroups of constituents co-purified with the pore-forming GluA1-4 proteins in anti-FRRS1l and anti-TARP APs highlighted by red boxes. Annotations on the right reflect molecular architecture and/or subcellular localization reported in literature or public databases. (b) Relative amounts of AMPAR constituents determined in two-step APs schematized on the right from CL-47 solubilized membrane fractions of total adult rat brains. Bars in red and blue depict the amount of AMPAR constituents in a target-depleting anti-FRRS1l AP (red bars, mean±s.d. of three measurements) and a subsequent target-depleting anti-GluA AP (blue), each determined as fraction of its summed protein amounts in the two APs. Brown bars illustrate relative amounts of AMPAR constituents (mean±s.d. of three measurements) in an anti-FRRS1l AP using the flow through of a target-depleting anti-GluA AP as input divided by the summed protein amount determined for any constituent in both APs from rat membranes. Note co-assembly of FRRS1l with GluA1-4 and only a subset of AMPAR proteome constituents.
