Figure 2: Specific co-assembly of FRRS1l and CPT1c into AMPAR complexes with distinct subunit composition.
From: AMPA-receptor specific biogenesis complexes control synaptic transmission and intellectual ability
(a) Amounts of proteins specifically co-purified in APs with four different anti-FRRS1l ABs (upper panel) and two different anti-CPT1c ABs (lower panel) normalized to the amount of purified FRRS1l or CPT1c, respectively (red bar). Data are mean±s.d. of five (four ABs, one AB mixture, see Methods section) and three (two ABs, one AB mixture) independent APs, respectively. (b) Protein abundance ratios (mean±s.e.m. of four measurements) determined for the indicated proteome constituents in anti-GluA1-4 APs from brain membrane fractions prepared from both WT and CPT1c knockout mice (normalized to GluA1-4). Note the pronounced decrease of CPT1c, FRRS1l and Sac1 in AMPARs from knockout animals. Dashed line indicating 50% reduction in protein amount is shown for orientation; asterisks denote statistical significance for protein amounts being different between WT and KO (**, ***P values of 0.01 and 0.001 for Students’ t-test, respectively).
