Figure 5: Subcellular localization of FRRS1l in neurons of the hippocampus.
From: AMPA-receptor specific biogenesis complexes control synaptic transmission and intellectual ability
(a) Confocal images of the hilar region of hippocampal slices prepared from adult rats and selected neurons therein triple stained with anti-FRRS1l, the ER-marker anti-Calnexin and the synapse marker anti-vGlut1. Graphs in the lower panel are fluorescence intensity scans of the annotated markers measured along the indicated lines on the confocal images of the selected neurons. (b) Electron micrographs showing immuno-reactivity for FRRS1l in somata (S) of CA1 pyramidal cells as detected by the pre-embedding immunogold method. Immunoparticles labelling FRRS1l were abundant over the endoplasmic reticulum (ER, arrows) but not on membranes of the Golgi complex (GC in lower panel). Particles (open arrowheads) occasionally appeared close to or at the somatic plasma membrane (PM, red line) targeted by boutons (b) of putative GABAergic neurons. Scale bar: 200 nm.
