Figure 7: Decrease or increase of currents mediated by synaptic and extra-synaptic AMPARs upon knockdown and exogenous (over)expression of FRRS1l in distinct types of hippocampal neurons.

(a) Amplitude histograms determined from spontaneous EPSCs recorded in uninfected control or sh-FRRS1l-transduced MCs and in MCs with virally driven overexpression of FRRS1l (at −70 mV). Data are mean±s.e.m. of 129 (control), 68 (sh-FRRS1l) and 10 (overexpression) MCs. Inset: EPSCs from a control and a sh-FRRS1l-transduced MC. Current and time scaling as indicated. (b) Summary plot of EPSC amplitudes determined in MCs, interneurons (INs) and CA3 pyramidal cells (CA3 PCs) under control conditions (uninfected) or after transduction with the sh-RNAs indicated on the upper right. Data are mean±s.e.m. of 7–135 MCs, 11–77 INs and 18–61 CA3 PCs; EPSC amplitudes were normalized to the mean amplitude of the control cells. Note that reduction and increase in EPSC amplitude induced by knockdown and overexpression of FRRS1l occurred in all three types of hippocampal neurons. (c) Representative spontaneous EPSCs (left) and glutamate-evoked currents in a somatic outside-out (oo) patch both recorded successively either in an uninfected MC (control, black traces) or an MC transduced with sh-FRRS1l (red traces) in a hippocampal slice preparation. Current and time scaling as indicated. (d) Summary plot of relative amplitudes obtained for EPSCs and glutamate-evoked AMPAR currents in control MCs and MCs transduced with the indicated sh-RNAs. Data are mean±s.e.m. of 8 MCs for each condition.