Figure 4: PDGFRβ regulates APC niche interaction and WAT niche expansion.
(a) Illustration of genetic alleles used to generate AT- and SMA-CreERT2-PDGFRβ loss (AT-PDGFRβ-LOF or SMA-PDGFRβ-LOF) and constitutively active PDGFRβ (AT-PDGFRβ-CA or SMA-PDGFRβ-CA) mice. Experiments were performed three times on 10 mice per group. (b) Representative H&E images of IGW adipose depots from mice described in a. (c) Representative histological sections from mice described in a stained for GFP, CD31 and SMA. (d) Representative images of SVPs isolated from mice described in a. (e) Representative images of vascular sprouts of subcutaneous IGW explants from the mice described in a. (f,g) Sprout length and branching points were quantified from explants described in e. (h) mRNA expression of vasculogenic genes from mice described in a. Data are expressed as means±s.e.m. Scale bars 100 μm. *P<0.01 unpaired t-test, two tailed: mutant compared to control levels. #P<0.001 unpaired t-test, two tailed: mutant PDGF-B treated compared to control PDGF-B treated.
