Figure 5: PDGFRβ restores APC niche interaction and expansion in AT-PPARγ-LOF mice. | Nature Communications

Figure 5: PDGFRβ restores APC niche interaction and expansion in AT-PPARγ-LOF mice.

From: A PPARγ transcriptional cascade directs adipose progenitor cell-niche interaction and niche expansion

Figure 5

(a) Illustration of genetic alleles used to generate AT-control, AT-PPARγ-LOF and AT-PPARγ-LOF-PDGFRβ-CA mice. At 2 months of age, mice were analysed. Experiments were performed three times on 10 mice per group. (b) Representative images of subcutaneous IGW depots stained for CD31, SMA and APC-GFP from mice described in a. (c) Representative image of SVPs isolated from mice described in a and visualized for GFP locality. (d) Representative images of vascular sprouts from subcutaneous IGW WAT explants excised from mice described in a. (e) Quantification of APC-GFP number per 100-micron SVP. (f) Quantification of sprout length of explants described in d. (g) Quantitative RT–PCR analysis of vasculogenic gene expression from mice described in a. Data are expressed as means±s.e.m. Scale bars 100 μm. *P<0.05 unpaired t-test, two tailed: mutant compared to control levels.

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