Figure 4: Theophylline-responsive base editing on the HEK-3 and FANCF loci in human cells.
From: Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
(a,b) Nuclease-mediated indel formation by HHR-bsgRNA and dHHR-bsgRNA on the HEK-3 (a) and FANCF (b) sites in the presence of Cas9. The protospacer sequences of both sites are shown with the protospacer adjacent motif (PAM) in italics. The base editing target bases are shown in magenta. (c,d) Base editing by HHR-bsgRNA and dHHR-bsgRNA on the HEK-3 (c) and FANCF (d) sites in the presence of BE3. (e) Theophylline-dependent base editing on the FANCF site. (f–h) Theophylline-dependent base editing on the HEK-3 site with different doses of agRNA and BE3 plasmids. Values and error bars reflect mean editing percentage and the s.d. of three biological replicates except the dHHR-bsgRNA and HHR-bsgRNA columns in panel e and the theophylline treated samples in e–h, for which two biological replicates were performed.
