Figure 1: Overview of the electrical cell-counting technology.
(a) Process schematic of the differential expression-based cell-counting technology. Whole blood (10 μl) is infused in the biochip along with lysing and quenching buffers, to preferentially lyse erythrocytes. Cells are electrically counted and differentiated based on their size using microfabricated electrodes. Anti-CD64 (clone 10.1) antibody is initially adsorbed in the chamber. The CD64+ cells get captured based on their CD64 expression level. The difference in the cell counts from cell counters is linearly correlated with the nCD64 expression level. (b) The resulting pulse amplitude histogram representing lymphocytes and granulocytes + monocytes as two distinct populations. (c) Correlation (coefficient of determination: R2=0.89, P<0.0001) in between biochip total leukocytes versus control leukocyte counts obtained from haematology analyzer using n=181 blood samples. (d) The correlation (coefficient of determination: R2=0.88, P<0.0001) in between biochip total granulocytes + monocytes versus control cell counts obtained from haematology analyzer.
