Figure 4: Patient stratification and time course measurements.

(a) The comparison plot between nCD64 value obtained from biochip versus flow cytometry, showing the good linear correlation of coefficient of determination: R²=0.87, P<0.0001. (b) The Bland–Altman analysis comparing the nCD64 values from biochip versus flow cytometer (control). It shows −0.0002 average difference of nCD64 value in between biochip and flow cytometer (control) and 0.49 value as limits of agreement. (c) ROC curves to predict the three bins used for sepsis diagnosis (Fig. 2h). AUC for Bins 1, 2 and 3 are 0.98, 0.85 and 0.9, respectively, showing the high predictability accuracy for all the bins. (d) The plot represents the nCD64 prediction accuracy from the biochip. The accuracy is > 85% for the bin size of 0.6 nCD64 value, which corresponds to ten bins. (e) The box plots showing the nCD64 expression value obtained from biochip from 91 blood samples collected from patients at different times of their hospital stay. (f) The box plots showing the total leukocyte counts obtained from biochip from 91 blood samples from the same patients as in e. (g) The plot representing the nCD64 value and total WBC counts obtained from biochip using 91 blood samples collected at different times of patient hospital stay. (h) Total leukocyte count and nCD64 value of the patients are tracked over time. (i) ROC curves showing high sensitivity and specificity of leukocytosis and neutrophilia diagnosis from the biochip.