Figure 6: Quantification of ternary complex assembly in living cells. | Nature Communications

Figure 6: Quantification of ternary complex assembly in living cells.

From: Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments

Figure 6

(a,b) Spatial correlation of TMRIL-13Rα1 and DY647IL-4Rα molecules for a representative single cell in absence (a) and presence (b) of IL-4, analysed by PICCS. Black circles: cumulative correlation function Ccum obtained from single molecule localizations, averaged over frames 1–20. Dashed green line: linear contribution of the cumulative correlation function (equation (1)). Orange dashed line: cumulative correlation function after subtraction of the linear term from the fitted function (equation (2), red curve). (c) Correlated fraction of TMRIL-13Rα1 and DY647IL-4Rα for the examined ligands, determined by PICCS. (d) Quantification of receptor dimerization by PICCS analysis of DY647IL-4 bound to TMRIL-4Rα and to TMRIL-13Rα1, respectively. Data are based on n=16 cells (IL-13Rα1+IL-4) and n=9 cells (IL-4Rα+IL-4). (e) The correlation length (σ) obtained from PICCS analysis. The data shown in c,e are pooled from at least two independent experiments with n>20 cells (n=9 cells for unstim. and n=6 cells for DN and pos. ctrl.). (f) Receptor distance distributions of co-trajectories, induced by KFR, IL-4, IL-13 and RGA, fitted with a three-component log-normal function. Depicted curves are based on data from different cells comprising n>50 trajectories with minimum length of 30 frames. Statistical analysis by a two-sample Kolmogorov–Smirnov test (*P<0.05).

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