Figure 8: Simulation of spatiotemporal receptor dynamics including MSK-dependent membrane compartmentation and hop-diffusion.

(a) Representative receptor distance distributions of co-trajectories as well as (b) the corresponding correlation lengths, simulated on the basis of the depicted model depicted in Supplementary Fig. 20 with two different ternary complex dissociation constants (n=5) are comparable to the experimentally derived data and verify the adopted model. (c) The mean ternary complex lifetime is increased by ∼50% in simulation runs including confinement by the MSK (mean±s.d., n=10). (d) Mechanistic model for ligand-induced type II interleukin-4 receptor dimerization sustained by rapid re-association within MSK-dependent TCZ. After two-step receptor assembly of the ternary complex (I), downstream signalling is initiated (II) that is maintained during transient dissociation events (III, IV) until receptor uptake by endocytosis (V).