Figure 1: ERG regulates endothelial Notch signalling. | Nature Communications

Figure 1: ERG regulates endothelial Notch signalling.

From: The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability

Figure 1

(a) Western blot (WB) analysis of Notch intracellular domain (NICD) expression in control (siCtrl) and ERG-deficient (siERG) HUVEC (n=4). (b) RBP-J TP-1 Notch reporter activity in control and ERG-deficient HUVEC (n=4). (c) qPCR of Notch target gene expression in siCtrl and siERG-treated HUVEC: Hes1, Hey1, Nrarp and p21 (n=4). (d) Microarray and PCR screen analysis of differential gene expression in HUVEC was performed at 24 and 48 h after ERG inhibition29, with fold change of selected genes represented as high (red) and low (blue) expression compared to the median (grey). (e) qPCR analysis of Hes1 and Hey1 Notch target gene expression in siCtrl and siERG-transfected HUVEC stimulated with Dll4 or control BSA (n=4). (f) RBP-J TP-1 Notch reporter activity in control and ERG-deficient HUVEC plated on Dll4 or BSA (n=4). (g) WB analysis and quantification of NICD expression in siCtrl and siERG-transfected HUVEC stimulated with Dll4 or BSA (n=4). (h) qPCR analysis of Notch1 and Notch4 mRNA expression in siCtrl and siERG-transfected HUVEC stimulated with Dll4 or BSA (n=4). All graphical data are mean±s.e.m., *P<0.05, **P<0.01, ***P<0.001, Student’s t-test.

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