Figure 4: ERG is required for Ang1 regulation of Dll4 and Jag1 expression and Notch signalling. | Nature Communications

Figure 4: ERG is required for Ang1 regulation of Dll4 and Jag1 expression and Notch signalling.

From: The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability

Figure 4

(a) Ang1-induced Notch transcriptional activity was determined by transfecting control and ERG-deficient cells with RBP-J TP1 reporter construct in the presence or absence of Ang1 (250 ng ml−1 for 6 h) (n=3). (b) Ang1-induced transcription of downstream Notch target gene Hey1 in control and ERG-deficient HUVEC (n=4). (c) qPCR and (d) WB analysis of Dll4 expression in extracts of siCtrl and siERG HUVEC treated in the presence or absence of Ang1 (250 ng ml−1 for 6 h) (n=5). (e) Jag1 mRNA and (f) protein expression in siCtrl and siERG HUVEC treated in the presence or absence of Ang1 (250 ng ml−1 for 6 h) (n=5). (g) Model: ERG mediates Ang1-dependent reciprocal regulation of Dll4 and Jag1 in endothelial cells. All graphical data are mean±s.e.m., *P<0.05, **P<0.01, ***P<0.001, Student's t-test.

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