Figure 2: Assessment of autophagy in mice with Raptor deletion in β-cells.
From: Loss of mTORC1 signalling impairs β-cell homeostasis and insulin processing
(a) Electron microscopy images (left) of islets from raptorf/f and βraKO mice at 30 days of age. Lysosomes containing insulin granules (crinophagy; arrows) are observed in β-cells from βraKO mice (inset shows magnification of crinophagy). Magnification: × 10,500. Images are representative of islets from three animals per group. Quantification of the number of multigranular bodies containing secretory granules per cell (right). (b) Electron microscopy images of multigranular bodies containing insulin secretory granules in βraKO mice at different magnifications. Scale bar, 1 μM in (a) and 300 nM in b. (c) Immunostaining for insulin (red), LC3-GFP (green) and DAPI (blue) in dispersed islets from raptorf/f and βraKO mice at 40 days of age. Scale bar, 10 μM. Percentage of cells with LC3-GFP puncta formation (arrows) in dispersed cells from LC3-GFP (raptorf/f and βraKO) mice at 40 days (n=4). Data are shown as means±s.e.m., *P<0.05; nonparametric U-test (Mann–Whitney).
