Figure 7: 4E-BP2 controls CPE levels by modulation of cap-dependent translation.
From: Loss of mTORC1 signalling impairs β-cell homeostasis and insulin processing
(a) Immunoblotting and quantification of CPE and actin in 4E-BP2 knockdown (sh4ebp2) and control MIN6 cells (shCtrl) treated or not with 4E1RCat (25 μm) for 24 h (n=6). (b) CPE expression at the mRNA level by RT–PCR in control and in sh4ebp2 cells (n=4). (c) Polyribosome profile of control and sh4ebp2 cells cultured in regular conditions (n=4). (d) CPE mRNA levels in polysomal and monosomal fractions from control and sh4ebp2 cells cultured in normal conditions. Data expressed as a ratio of CPE expression in polysome/monosome (P/M) fractions (n=4). (e) Immunoblotting and quantification of CPE and actin in AtT20 cells treated or not with 4E1RCat for 24 h (n=4). (f) Immunoblotting and quantification of POMC, αMSH and actin in AtT20 cells treated or not with 4E1RCat for 24 h (n=4). Data are shown as means±s.e.m., *P<0.05; nonparametric U-test (Mann–Whitney).
