Figure 2: Internalized FZD4 is targeted to the endosome and lysosome. | Nature Communications

Figure 2: Internalized FZD4 is targeted to the endosome and lysosome.

From: Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function

Figure 2

(a) 30 min after Norrin-induced internalization at 37 °C FZD4 co-localizes with the early endosomal marker EEA1 in HeLa cells. (b) No substantial co-localization with the recycling endosome marker Rab11 was detected. (c) FZD4 co-localizes with the lysosomal marker LAMP1 and (d) RAB7. Co-localization was quantified using the Pearson correlation coefficient. Mean+s.d. shown, n=20. In a–d internalized FZD4 is specifically revealed by co-internalized V5-antibody. (e,f) Triple staining for FLAG-AP-Norrin, V5-FZD4 and markers for membrane trafficking excited in the 488 nm (green), 555 nm (red) and 647 nm (magenta) channels. Boxed areas are shown enlarged on the right. The DAPI channel was omitted in the enlarged 3-colour merge. Norrin and FZD4 traffic together into the early endosome and lysosome. Arrowheads show examples of co-localization. Scale bars, 10 μm.

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