Figure 3: Effect of DVL2 mutations on FZD4 endocytosis. | Nature Communications

Figure 3: Effect of DVL2 mutations on FZD4 endocytosis.

From: Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function

Figure 3

(a) WNT5A/PMA-induced FZD4 internalization in HeLa cells. WT DVL2 binds and co-localizes with FZD4 and does not form cytoplasmic aggregates. (b) DVL2 K466M is FZD4-binding deficient and thus forms cytoplasmic aggregates. In the DVL2 K466M expressing cells, WNT5A/PMA fails to induce FZD4 endocytosis. (c) DVL2 AHEL, which is impaired in AP2 binding, does not form cytoplasmic aggregates but also prevents WNT5A/PMA-induced internalization of FZD4. (d–f) NDP induces strong FZD4 endocytosis regardless of K466M or AHEL mutations in co-expressed DVL2. Scale bar, 10 μm.

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