Figure 2: Nucleolar size inversely correlates with longevity.

(a) eat-2(ad465) animals have smaller nucleoli while ncl-1(e1942) and eat-2;ncl-1 animals possess larger nucleoli compared to N2. (20 worms imaged per replicate, 3 independent biological replicates) (b) Nucleolar size is reduced on bacterial food reduction with a corresponding increase in lifespan. (P<0.0001, log-rank test). (c,d) eat-2(ad465), TOR RNAi, isp-1(qm150), glp-1(e2141) and daf-2(e1370) animals possess smaller nucleoli while daf-2;daf-16 have nucleoli similar to N2 in the hypodermis and pharyngeal muscle (Error bars represent mean±s.d.) (e,f) Schematic illustrating the experiment, which shows that longer-lived worms exhibit small nucleoli and vice versa. (The graph depicts mean and s.d. Pearson correlation coefficient R²=0.93 is calculated using the entire data set, the line equation is y=−0.1483x+6.9148). Scale bar represents 5 μm. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, NS, non-significant, unpaired t-test.