Figure 3: Kinetics of RT reaction with GSH.
(a) RT reacts rapidly with GSH in both forward and reverse reactions. All data were recorded at 37 °C. The kinetic traces for reverse reactions were shifted on x axis for clarity. (b) Catalytic effect of GST on the reaction rate between GSH and RT. Data points represent the fluorescence signal from newly generated RT-GSH conjugate. No significant differences can be observed with or without GST. One representative set of data out of three independent experiments are shown.
