Figure 6: Comparison of GSH quantifications using RT probe and lysate-based measurements.
(a) A BSO concentration-dependent decrease of GSH levels measured using FACS. HeLa cells treated with a series of concentrations (0.9–500 μM) of BSO for 72 h were harvested and analysed using RT. R=F405 nm/F488 nm; Rmin and Rmax are the corresponding R values with 0 and saturating GSH concentrations, respectively. Each data point represents the mean value of 17,000–28,000 cells analysed from three independent experiments. Error bars represent s.d. (b) Correlation of GSH levels in BSO-treated HeLa cells measured using the RT-based flow cytometry method in a and a lysate-based biochemical assay. Each point in the lysate measurements represents the mean value of a total of nine replicates from three independent experiments. Error bars represent s.d.
