Table 1 Data collection, refinement parameters and model statistics.
From: Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate
Data collection | |
Particles used in final 3D refinement | 175,374 |
Pixel size (Å) | 0.525 |
Defocus (μm) | −0.5 |
Voltage (kV) | 300 |
Electron dose (e−Å−2) | 40 |
R.m.s. deviations | |
Bonds (Å) | 0.01 |
Angles (°) | 1.53 |
Validation | |
Clashcore, all atoms | 3.53 |
Good outliers (%) | 0.0 |
Ramachandran plot | |
Favoured (%) | 95.23 |
Allowed (%) | 4.77 |
Outliers (%) | 0.0 |
Refinement of C2 symmetry map (175,374 particles) | |
Resolution (Å) | 3.2 |
Map sharpening B-factor (Å2) | −176 |
Fourier shell correlation criterion | 0.143 |
Refinement of asymmetric map (175,374 particles) | |
Resolution (Å) | 3.4 |
Map sharpening B-factor (Å2) | −179 |
Fourier shell correlation criterion | 0.143 |
Refinement of asymmetric map (76,150 particles) | |
Resolution (Å) | 3.6 |
Map sharpening B-factor (Å2) | −157 |
Fourier shell correlation criterion | 0.143 |
