Figure 4: Evolution of the extension strength of the wrist in the forelimbs of GRMD dogs injected with rAAV2/8-Spc5.12-cMD1 by the LR route.

The extension strength of the wrist of the forelimbs was measured using a specific torque measurement device. Three measurement sessions were performed all along the protocol: before injection, at day +45 and at day +90. (a) The evolution of the maximal torques, normalized by the animal weight, over the three different measurement sessions, was represented on each panel. Each point represents the mean value (±95% confidence interval) of the results obtained in forelimbs of healthy (WT) golden retriever dogs (n=14, grey line), in forelimbs of untreated GRMD dogs (n=6, grey dotted line), in the injected forelimb of rAAV2/8-Spc5.12-cMD1-treated GRMD dogs (n=4, dark line) and in the noninjected forelimb of the same dogs (n=4, dark dotted line). (b) Extension strength change between day 0 (before injection) and day +90. Each point represents the ratio between the maximal torque (normalized by the animal weight) obtained at day 0 and at day +90 in a same forelimb for healthy (WT) golden retriever dogs (n=14, grey full symbols), in untreated GRMD dogs (n=6, grey empty line), in the injected forelimb of rAAV2/8-Spc5.12-cMD1-treated GRMD dogs (n=4, black full symbols) and in the noninjected forelimb of the same dogs (n=4, black empty symbols). The horizontal bars represent the mean of the values obtained for each group. *P<0.05 (nonparametric Kruskal–Wallis test with post hoc multiple comparison Dunn’s test).