Figure 3: In vivo functional characterization of lmbA and its orthologue lmbA2991. | Nature Communications

Figure 3: In vivo functional characterization of lmbA and its orthologue lmbA2991.

From: 4-alkyl-L-(Dehydro)proline biosynthesis in actinobacteria involves N-terminal nucleophile-hydrolase activity of γ-glutamyltranspeptidase homolog for C-C bond cleavage

Figure 3

(a) Product profiles of S. lincolnensis strains, including the wild-type control (I) and mutants (II, for the ΔlmbA single mutation; III, for homologous complementation of the ΔlmbA mutant by lmbA; IV, for heterologous complementation of the ΔlmbA mutant by ant6; V, for ΔlmbA-lmbA2991 double mutation; VI, for the ΔlmbD single mutation). (b) The assembly of PPL and 6 that is mediated by a discrete NRPS system involving LmbD activity.

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