Figure 4: Analysis of autoproteolytic activity on SDS–PAGE gels. | Nature Communications

Figure 4: Analysis of autoproteolytic activity on SDS–PAGE gels.

From: 4-alkyl-L-(Dehydro)proline biosynthesis in actinobacteria involves N-terminal nucleophile-hydrolase activity of γ-glutamyltranspeptidase homolog for C-C bond cleavage

Figure 4

The asterisk indicates an unknown impure protein associated with the purification of γ-GT homologues/variants. (a) Ant6. Lane 1, protein standard; Lane 2, Ant6 wild-type; Lane 3, Ant6-T430S; Lane 4, Ant6-T430C; Lane 5, Ant6-D429A&T430A; Lane 6, Ant6-T430A; Lane 7, Ant6-D429A. (b) LmbA2991. Lane 1, protein standard; Lane 2, LmbA2991 wild-type; Lane 3, LmbA2991-D420A; Lane 4, LmbA2991-T421A; Lane 5, LmbA2991-D420A&T421A; Lane 6, LmbA2991-T421C; Lane 7, LmbA2991 T421S. (c) Reconstituted LmbA2991 heterodimer (HD) large (L) and small (S). Lane 1, protein standard; Lane 2, heterodimer wild-type; Lane 3, LmbA2991 HD L-D420A&S; Lane 4, LmbA2991 HD L&S-T1A; Lane 5, LmbA2991 HD L&S-T1C; Lane 6, LmbA2991 HD L&S-T1S.

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