Figure 7: L–R patterning is determined by the number of motile cilia in Dpcd mutant embryos.

(a–h) Relation between the position of one motile cilium and L–R asymmetric gene expression in Dpcd mutant embryos at the four-to-six-somite stages. (i–l) Relation between the position of two motile cilia and L–R asymmetric gene expression in Dpcd mutant embryos at the nine and the six-somite stages. (m,n) Relation between the position of motile cilia and L–R asymmetric gene expression in a wild-type (+/+) embryo at the six-somite stage. Coloured circles indicate rotating cilia, with red and pink circles denoting posteriorly tilted cilia; green and white circles denote rotating cilia showing no tilt. Although all the rotating cilia are shown for the mutant embryos (a,c,e,g,i,k), only a portion of rotating cilia detected on a single plane is shown for the wild-type (+/+) embryos (m). Cyan dots denote immotile cilia in the perinodal region. The white, black and red scale size bars indicate 10 μm, 50 μm and 0.5 mm, respectively.