Figure 5: ACVRL1 and EFNB2 show interdependent expression with PTPN14 in vitro. (a–f) HUAECs were treated with indicated siRNAs, and cultured for 2 days. | Nature Communications

Figure 5: ACVRL1 and EFNB2 show interdependent expression with PTPN14 in vitro. (a–f) HUAECs were treated with indicated siRNAs, and cultured for 2 days.

From: Mouse and human strategies identify PTPN14 as a modifier of angiogenesis and hereditary haemorrhagic telangiectasia

Figure 5

Quantitative reverse transcription–PCR (a,c,e) and western blot analysis (b,d,f) were then performed for EFNB2/EphrinB2 (a–d) and PTPN14 (e,f). (g) Cartoon summary of possible interactions implied by data in (a–f); PTPN14 downregulation by ACVRL1 may be directly or indirectly mediated via elevated EFNB2 levels. Data in panels a, c and e are representative of at least three independent biological replicates. Data in panels b, d and f are representative of two independent biological replicates. Moreover, data in panels b, d and f are from additional experiments, independent from those shown in a, c and e. Mean and s.d. are shown. ***P<0.0001, **P<0.001, *P<0.01, Students t-test.

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