Figure 2: Immunoscope profiles from CD4+ T-cell lines expanded with Flu1 or Flu2 (Arg at P10). | Nature Communications

Figure 2: Immunoscope profiles from CD4+ T-cell lines expanded with Flu1 or Flu2 (Arg at P10).

From: Modification of the carboxy-terminal flanking region of a universal influenza epitope alters CD4+ T-cell repertoire selection

Figure 2

Short-term T-cell lines were generated from peripheral blood stimulated with either Flu1 or Flu2. Antigen-specific CD4+ cells were isolated on the basis of HLA-DR1/HA305−320 tetramer staining, and TCR repertoires were assessed by spectratyping. The profiles of pooled CDR3β lengths for four TRBV families are shown. In peripheral blood, the expected Gaussian distribution is observed. Altered profiles for the Flu1-specific and Flu2-specific populations indicate oligoclonal expansions. Differences in TRBV family usage are also apparent, suggesting that particular clonotypic expansions can be driven by a C-terminally modified peptide. A representative example from three independent experiments is shown.

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