Figure 1: Akt2 controls expression of specific glycolytic isozymes in PTEN-null liver.
From: PPARγ contributes to PKM2 and HK2 expression in fatty liver
Relative transcript (a) and protein (b) levels of glycolytic enzymes in the mouse livers of indicated genotypes. For protein analysis, the ratio to β-actin of the densitometric assay is presented. Data are mean±s.e.m., n=3–7 (P<0.05 (a) versus WT; (b) versus AlbCre;PTENf/f; 2-tailed, unpaired Student's t test). (c) RT–QPCR for PKM2 and HK2 expression from mice livers of indicated genotypes. Data are mean ±s.e.m., n=7 (P<0.05 (a) versus WT; (b) versus AlbCre;PTENf/f; 2-tailed, unpaired Student's t test). (d) Immunoblot analysis of HK2 in liver mitochondria extracts from mice of indicated genotypes. The ratio to VDAC of the densitometric assay ±s.e.m. is presented, n=3–4 (P<0.05 (a) versus WT; (b) versus AlbCre;PTENf/f; 2-tailed, unpaired Student's t test). (e) Hepatic lactate levels of random-fed mice of indicated genotypes. Data are mean ±s.e.m., n=5–8 (P<0.05 (a) versus WT; (b) versus AlbCre;PTENf/f; 2-tailed, unpaired Student's t test).
